Interactive Multivariable qPCR Simulator By Filippo D. Galbo

Number of Targets: 2

Max Cycles: 40

Fluorescence Threshold (Ct): 10k

Adjust slider or drag line on fluorescence graph after run.

Max Fluorescence (Saturation): 100k

Subtract Calculated Baseline (Cycles 3-10)

Ideal: 95°C, 60°C, 72°C. Deviations reduce max efficiency.

Denaturation: 95 °C

Annealing: 60 °C

Extension: 72 °C

Temp Penalty Factor: 1.00

Simulates inhibitors affecting the overall reaction efficiency.

Inhibitor Type:

Inhibitor Level: 0

Inhibitor Penalty Factor: 1.00

Status: Idle